Cryogenic distillation is the most common method of removing nitrogen from natural gas. Homogeneous isotopic exchange is caused by the existence of admixtures in NO and can spontaneously occur in the entire volume of the distillation columns. Cryogenic Distillation is an effective method used for isotope separation. The models for simulation of this distillation use the concept of theoretical plate that simultaneously brings to thermodynamic equilibrium to all the components in a multi-component mixture. It is highly energy intensive. The separation energy for cryogenic distillation in the production of natural gas and ethylene is greater than total energy requirements. This distillation requires two phases like gas and liquid. This process can separate tritium from both heavy and light water.